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轉(zhuǎn)鐵蛋白

    BR,98%

TRF

源葉
S12027
11096-37-0
MFCD00131337
鐵傳遞蛋白
品牌 貨號(hào) 產(chǎn)品規(guī)格 價(jià)格(RMB) 庫(kù)存(上海) 北京 武漢 南京 購(gòu)買數(shù)量
源葉 S12027-10mg BR,98% ¥120.00元 4 - 1 3
源葉 S12027-50mg BR,98% ¥380.00元 >10 1 - 2
源葉 S12027-100mg BR,98% ¥700.00元 >10 2 6 1
源葉 S12027-500mg BR,98% ¥2800.00元 4 1 - 3
產(chǎn)品介紹 參考文獻(xiàn)(51篇) 質(zhì)檢證書(shū)(COA) 摩爾濃度計(jì)算器 相關(guān)產(chǎn)品

產(chǎn)品介紹

介紹:轉(zhuǎn)鐵蛋白是血漿中主要的鐵傳遞蛋白,為細(xì)胞分化和細(xì)胞代謝提供所需的鐵。研究發(fā)現(xiàn) 所有的細(xì)胞生長(zhǎng)都離不開(kāi)轉(zhuǎn)鐵蛋白。不但維持正常的細(xì)胞代謝依賴于以生物活性形態(tài)存在的 鐵,鐵還是一些酶的輔助因子,比如 RNA 聚合酶,DNA 合成酶。同時(shí)也是血紅蛋白的重要 組成部分。 轉(zhuǎn)鐵蛋白是血漿糖蛋白,從血漿中分離而來(lái),牛轉(zhuǎn)鐵蛋白以兩種分子形態(tài)存在,分子量 分別為 74KD 和 78KD。每個(gè)轉(zhuǎn)鐵蛋白分子通過(guò) C 端和 N 端結(jié)構(gòu)位點(diǎn)可以結(jié)合兩分子鐵離子, 所以轉(zhuǎn)鐵蛋白以三種形態(tài)存在:脫鐵(不結(jié)合鐵離子),飽和鐵(結(jié)合兩分子鐵離子)和部 分飽和,在生理情況下,轉(zhuǎn)鐵蛋白分子僅有 1/3 被鐵飽和。 使用說(shuō)明: 轉(zhuǎn)鐵蛋白對(duì)于絕大部分細(xì)胞系在無(wú)血清培養(yǎng)基中生長(zhǎng)是必需的,研究表明飽和鐵形態(tài)的 轉(zhuǎn)鐵蛋白對(duì)于促進(jìn)細(xì)胞生長(zhǎng)是最有效的形態(tài)。對(duì)于雜交瘤,黏連細(xì)胞和懸浮細(xì)胞系,在細(xì)胞 培養(yǎng)過(guò)程中,應(yīng)保持培養(yǎng)基中轉(zhuǎn)鐵蛋白濃度在 0.5-100μ g/ml。針對(duì)不同的細(xì)胞系,需要選 擇最合適的濃度。 轉(zhuǎn)鐵蛋白凍干粉需要保存在冷藏狀態(tài)下,使用之前再溶解。一般用 PBS 或者其它合適 的溶劑在室溫下將轉(zhuǎn)鐵蛋白凍干粉溶解成儲(chǔ)液,濃度在 0.5-2.0mg/ml,然后用無(wú)菌濾膜進(jìn)行過(guò) 濾,濾液可在無(wú)菌條件下 2-8℃保存 30 天。
外觀: 凍干粉末
儲(chǔ)存條件: 2-8℃
注意: 部分產(chǎn)品我司僅能提供部分信息,我司不保證所提供信息的權(quán)威性,僅供客戶參考交流研究之用。

參考文獻(xiàn)(51篇)

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摩爾濃度計(jì)算器

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