| 產(chǎn)品描述： | VE-822 has been used in trials studying the treatment of Ovarian Neoplasms, Ovarian Serous Tumor, Adult Solid Neoplasm, Advanced Solid Tumor, and Advanced Solid Neoplasm, among others. |
| 靶點(diǎn)： |
ATM/ATR |
| 體內(nèi)研究： |
80 nM VE-822單獨(dú)使用增加MiaPaCa-2和PSN-1細(xì)胞停留在G1期的比率。80 nM VE-822消除MiaPaCa-2和PSN-1細(xì)胞中富含XRT的G2/M期部分。VE-822單獨(dú)作用不大,而80 nM VE-822與XRT和/或gemcitabine聯(lián)用則增強(qiáng)PSN-1細(xì)胞中的早期和晚期細(xì)胞凋亡。VE-822增加對(duì)與pChk1 Ser345阻斷相關(guān)的DNA損傷劑的腫瘤應(yīng)答。VE-822（80 nM）減弱ATR信號(hào)傳導(dǎo)途徑并降低腫瘤細(xì)胞對(duì)XRT和吉西他濱的應(yīng)答的存活率。在正常細(xì)胞中,80 nM VE-822減弱ATR信號(hào)通路強(qiáng)度,但并沒有增強(qiáng)輻射和gemcitabine殺傷正常細(xì)胞的能力 |
| 細(xì)胞實(shí)驗(yàn)： |
VE-822 is dissolved in DMSO and stored, and then diluted with appropriate media before use. Gemcitabine (10 nM) is added 24 h pre-XRT and is replaced with fresh medium before addition of VE-822. PSN-1 cells are treated with VE-822 (80 nM) for 1 h before, through to 18 h after, XRT (6 Gy). Apoptosis is analyzed 48 h after XRT by flow cytometry using an Annexin V-FITC kit with Pl |
| 參考文獻(xiàn)： |
1.Fokas E, et al. Cancer Treat Rev, 2013, pii: S0305-7372(13)00065-0. 2.Fokas E, et al. Cell Death Dis, 2012, 3, e441. 3.Konstantinopoulos P A , Cheng S C , Hendrickson A E W , et al. Berzosertib plus gemcitabine versus gemcitabine alone in platinum-resistant high-grade serous ovarian cancer: a multicentre, open-label, randomised, phase 2 trial[J]. The Lancet Oncology, 2020, 21(7). |
| 溶解性： |
DMSO:34 mg/mL (73.3 mM) Ethanol:<1 mg/mL |
| 保存條件： |
2-8℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.157 ml |
10.786 ml |
21.573 ml |
| 5 mM |
0.431 ml |
2.157 ml |
4.315 ml |
| 10 mM |
0.216 ml |
1.079 ml |
2.157 ml |
| 50 mM |
0.043 ml |
0.216 ml |
0.431 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
上海工商
危險(xiǎn)品化學(xué)品經(jīng)營(yíng)許可證（不帶存儲(chǔ)）
營(yíng)業(yè)執(zhí)照(三證合一)
北京