| 產(chǎn)品描述： | ABT-751 has been investigated for the treatment of Lung Cancer, Non-Small Cell Lung Cancer, and Non-Small-Cell Lung Cancer. |
| 靶點(diǎn)： |
Autophagy Microtubule Associated;MicrotubuleAssociated;Autophagy |
| 體內(nèi)研究： |
ABT-751顯示出對(duì)動(dòng)態(tài)微管的選擇性作用并且保留穩(wěn)定的微管,從而解釋了在ABT-751的IC90濃度下乙酰化和去酪氨酸α-微管蛋白陽(yáng)性聚合小管的持久性。在體外,ABT-751顯示選擇性細(xì)胞毒性,在神經(jīng)母細(xì)胞瘤中IC50為0.6-2.6 μM,在其他實(shí)體瘤細(xì)胞系中為0.7-4.6 μM |
| 細(xì)胞實(shí)驗(yàn)： |
Cells, in 1640 RPMI media with FBS, are plated in triplicate onto 96 well tissue culture plates in numbers determined optimal for confluent monolayer growth (5,000 cells/well for HOS, HTB-186 Daoy; 10,000 cells/well for TC-71, RD, SK-N-AS, SK-N-DZ, LD; 30,000 cells/well for KCNR), with an automated, multichannel pipette system. Cells are incubated for 24 hours at 37 °C/5% CO2 then exposed to vehicle control (1.25% DMSO/Water), VCR (0.1–1000 nM), ABT-751 (0.1 nM–100 μM), and in 4 cell lines (SK-N-AS, KCNR, RD, TC-71) combretastatin (0.1–1000 nM) for 72 hours. Cells are fixed with trichloroacetic acid (final concentration 10%) at 4 °C, washed, then dried at room temperature, stained with SRB in 1% acetic acid and dye is then solubilized with Tris base. Optical density measurements are performed at 540 and 405 nm dual wavelengths in a Bio-Tek EL 340 UV plate reader. (Only for Reference) |
| 參考文獻(xiàn)： |
1.Meany HJ, et al. Pediatr Blood Cancer. 2010, 54(1), 47-54. 2.Jorgensen TJ, et al. Cancer Chemother Pharmacol. 2007, 59(6), 725-732. 3.Silver M, et al. J Vet Intern Med. 2012, 26(2), 349-354. |
| 溶解性： |
DMSO:37.1 mg/mL (100 mM) Ethanol:9.3 mg/mL (25 mM) |
| 保存條件： |
-20℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.692 ml |
13.462 ml |
26.924 ml |
| 5 mM |
0.538 ml |
2.692 ml |
5.385 ml |
| 10 mM |
0.269 ml |
1.346 ml |
2.692 ml |
| 50 mM |
0.054 ml |
0.269 ml |
0.538 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
上海工商
危險(xiǎn)品化學(xué)品經(jīng)營(yíng)許可證（不帶存儲(chǔ)）
營(yíng)業(yè)執(zhí)照(三證合一)
北京