| 產(chǎn)品描述： | EHT 1864 is a effective Rac family GTPase inhibitor for Rac1/Rac1b/Rac2/Rac3 (Kd: 40/50/60/250 nM). |
| 靶點： |
Ras;Rho;Rho;?Ras |
| 體內(nèi)研究： |
EHT 1864 (40 mg/kg i.p.) significantly reduces Abeta 40 and Abeta 42 levels in guinea pig brains. |
| 細胞實驗： |
NIH 3T3 cells stably expressing oncogenic Ras are plated in 96-well plates. The cells are cultured for up to 4 days in complete growth medium, either alone, or supplemented with 5 μM EHT 1864. Cell growth is then assessed using the conversion of MTT to a formazan product. Briefly, the MTT reagent (from a 5 mg/ml solution diluted in PBS) is added to the wells at a final concentration of 0.5 mg/ml, and the cells are further incubated for 4 h at 37°C. The medium is then removed, and the reaction is terminated by adding 100 μl/well Me2SO. The absorbance is read at 570 nm using a microplate reader.(Only for Reference) |
| 參考文獻： |
1.Shutes A, et al. J Biol Chem. 2007, 282(49), 35666-35678. 2.Raynaud F, et al. J Biol Chem. 2014, 289(5), 2600-2609. 3.Liu X, Wang S, Zheng H, et al. Epimedokoreanin C, A Prenylated Flavonoid Isolated from Epimedium Koreanum, Induces Non-Apoptotic Cell Death with the Characteristics of Methuosis in Lung Cancer Cells[J]. 2020 4.Désiré L, et al. J Biol Chem. 2005, 280(45), 37516-3752 |
| 溶解性： |
DMSO:43.6 mg/mL (75 mM) H2O:58.2 mg/mL (100 mM) |
| 保存條件： |
2-8℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.72 ml |
8.599 ml |
17.198 ml |
| 5 mM |
0.344 ml |
1.72 ml |
3.44 ml |
| 10 mM |
0.172 ml |
0.86 ml |
1.72 ml |
| 50 mM |
0.034 ml |
0.172 ml |
0.344 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。 |
上海工商
危險品化學品經(jīng)營許可證（不帶存儲）
營業(yè)執(zhí)照(三證合一)
北京