| 產(chǎn)品描述： | C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylicacid)是一種更穩(wěn)定的淺藍(lán)菌素合成模擬物，抑制脂肪酸合酶(fatty-acid synthase, FAS/FASN)，IC50為15.53 μM，在多種癌癥模型中具有潛在的治療作用。C75也是一種有效的CPT1A激活劑。 |
| 靶點(diǎn)： |
FAS/FASN:15.53 μM;FattyAcidSynthase |
| 體內(nèi)研究： |
C75, an alpha-methylene-gamma-butyrolactone, reduces adipose tissue and fatty liver in treated DIO mice, despite high levels of malonyl-CoA |
| 細(xì)胞實(shí)驗(yàn)： |
Cell lines: PC3, UVW and LNCaP cell lines, SK-N-BE(2c) and MCF7 cells Concentrations: 35 μM Incubation Time: 24 h Method: Cells are seeded in 25 cm2 flasks at 105 cells/flask. When cultures are in exponential growth phase, medium is removed and replaced with fresh medium (including 1% or 10% serum) containing C75 (35 mM) and simultaneous administration of x-rays (2 Gy). Cells are then incubated for 24 h at 37℃ in 5% CO2. After treatment, cells are counted and seeded for clonogenic survival assay. |
| 動(dòng)物實(shí)驗(yàn)： |
Animal Models: Diet-induced obese mice model Dosages: 20, 15, 10, 15, 15 mg/kg Administration: i.p. |
| 參考文獻(xiàn)： |
1. Wang X,et al. Bioorg Med Chem. 2009 Mar 1;17(5):1898-904. 2. Cheng X, et al. Org Lett. 2014 Mar 7;16(5):1414-7. 3. Rae C, et al. Adv Radiat Oncol. 2020 Jun 29;5(5):994-1005. 4. Thupari JN, et al. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9498-502. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存條件： |
2-8℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.932 ml |
19.66 ml |
39.321 ml |
| 5 mM |
0.786 ml |
3.932 ml |
7.864 ml |
| 10 mM |
0.393 ml |
1.966 ml |
3.932 ml |
| 50 mM |
0.079 ml |
0.393 ml |
0.786 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
上海工商
危險(xiǎn)品化學(xué)品經(jīng)營許可證（不帶存儲(chǔ)）
營業(yè)執(zhí)照(三證合一)
北京