| 產(chǎn)品描述： | STING agonist-1 (G10) 是一種新型人類特異性 STING 激動劑，可在人成纖維細(xì)胞中觸發(fā) IFN regulatory factor 3 (IRF3)/ type I interferon (IFN) 相關(guān)轉(zhuǎn)錄。STING agonist-1 (G10) 可有效抑制 Chikungunya virus (CHIKV) 的生長，IC90值為8.01 μM 和 Alphavirus species Venezuelan Equine Encephalitis Virus (VEEV)的復(fù)制，IC90為24.57 μM |
| 靶點(diǎn)： |
STING; IRF3/IFN; CHIKV(Cell-based assay):8.01 μM; VEEV(Cell-based assay):24.57 μM;VirusProtease;STING |
| 體外研究： |
G10 induces IFN/IRF3- but not NF-κB-dependent transcription in human fibroblasts. G10 elicits antiviral activity against New and Old World Alphaviruses. G10 induces STING-dependent synthesis and secretion of bioactive interferon. G10 triggers IRF3-dependent activity and induces innate antiviral mRNA expression in primary human cells. |
| 體內(nèi)研究： |
Because G10-induced innate immune activation is not observed in murine cells, DMXAA (an analogs of G10, with similar physiological effects) is used in the commenly used mice model of CHIKV infection. STING agonism inhibits CHIKV replication in vivo |
| 細(xì)胞實(shí)驗(yàn)： |
Cell lines: Human foreskin fibroblasts, Vero cells, BHK-21 cells, C6/36 cells, RAW264.7 cells, THP1-ISG-Lucia cells, Human peripheral blood mononuclear cells Concentrations: 6.25-200 μM, 100 μM Incubation Time: 7 h, 18 h Method: -- |
| 參考文獻(xiàn)： |
1. Sali TM, et al. Characterization of a Novel Human-Specific STING Agonist that Elicits Antiviral Activity Against Emerging Alphaviruses. PLoS Pathog. 2015 Dec 8;11(12):e1005324. |
| 溶解性： |
soluble in DMSO |
| 保存條件： |
-20℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.321 ml |
11.604 ml |
23.208 ml |
| 5 mM |
0.464 ml |
2.321 ml |
4.642 ml |
| 10 mM |
0.232 ml |
1.16 ml |
2.321 ml |
| 50 mM |
0.046 ml |
0.232 ml |
0.464 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
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危險品化學(xué)品經(jīng)營許可證（不帶存儲）
營業(yè)執(zhí)照(三證合一)
北京